Invitrogen superscript manual

Invitrogen Corporation, Faraday Avenue, Carlsbad, California Phone () Fax () ©–04 Invitrogen Corporation. All rights reserved. For research use only. Not intended for any animal or human therapeutic or diagnostic use. SuperScript™ III Reverse Transcriptase Cat. No. Size: 2, units. SuperScript® IV Reverse Transcriptase Protocol outline A. Anneal primer to RNA B. Assemble reaction mix C. Add reaction mix to annealed RNA RT reaction setup Use the measurements below to prepare your RT reaction, or enter your own parameters in the column provided. Component µL rxn Custom Final Conc. DEPC-treated water to 20 µL to µL N/AFile Size: 1MB. The 10X SuperScript™ Enzyme Mix includes SuperScript™ III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein. SuperScript™ III RT is an engineered version of M–MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°www.doorway.ru Size: KB.

E. coli RNaseH, Invitrogen Life Technologies, P/N T4 DNA Polymerase, Invitrogen Life Technologies, P/N 5X Second-strand buffer, Invitrogen Life Technologies, P/N 10 mM dNTP, Invitrogen Life Technologies, P/N M EDTA SuperScript Choice System contains, in addition to SuperScript II Reverse. Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit. Provides the high-temperature capability of SuperScript III Reverse Transcriptase (RT) in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR) Pricing and Availability. Invitrogen™ SuperScript™ IV VILO™ Master Mix with ezDNase. SuperScript ™ II Reverse Life Technologies Corporation will not assert a claim against the buyer of infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in.

The 10X SuperScript™ Enzyme Mix includes SuperScript™ III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein. SuperScript™ III RT is an engineered version of M–MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C. Fast, superior cDNA synthesis with SuperScript IV Reverse Transcriptase. The Invitrogen SuperScript line of reverse transcriptases (RTs) represents the most widely used and highly referenced RTs for cDNA synthesis in many applications. Invitrogen Superscript IV RT, the most recently introduced enzyme, was engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples. Superscript vilo cdna synthesis kit manual. Race kit (rapid amplification of cdna ends) is used for amplification of 3' or 5' of cdna. currently we are using the superscript iii first- strand synthesis kit from invitrogen. alternatively, superscript® vilo cdna synthesis kit and the related thermal protocol were employed. the presence of each indicated mrna was assessed in total rna samples by conventional rt- pcr using a superscript® vilo ™ cdna synthesis kit (invitrogen) and platinum.